Full-length cDNA sequencing of ampliconic genes from chimpanzee testi... - SRA

SRX18797582: Full-length cDNA sequencing of ampliconic genes from chimpanzee testis (8720_2 technical replicate 1)
1 PACBIO_SMRT (Sequel) run: 15,529 spots, 14M bases, 5.8Mb downloads

Design: Total RNA was extracted from testis sample using the RNeasy Mini Kit following the manufacturer recommended protocol (Qiagen, USA). Two cDNA technical replicates were generated with SMARTer PCR cDNA Synthesis Kit (Clontech, USA). These cDNAs were used as templates for a selective pulldown of polyadenylated ampliconic gene cDNAs using double hybridization of designed in-house biotinylated capture probes (a total of 10 probes, one for each of the nine ampliconic gene transcript families was used except for VCY gene cDNAs captured using human- and chimp/bonobo-specific probes).

Submitted by: Penn State University

Study: Full-length cDNA sequencing of ampliconic genes from great ape testis samples

PRJNA911852 • SRP412912 • All experiments • All runs

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The project consists of PacBio IsoSeq sequencing of full-length cDNAs of nine ampliconic gene families from eight great ape testis samples (2 humans, 2 chimpanzees, bonobo, gorilla, Bornean orangutan, and Sumatran orangutan). Two technical replicates of cDNA were generated from each of eight RNA samples. These cDNAs were used as templates for a selective pulldown of polyadenylated ampliconic gene cDNAs using double hybridization of each gene-family-specific capture probes. The project uses isONclust, a tool to cluster transcripts belonging to same gene families.

Sample: Testis sample from chimpanzee Pan troglodytes troglodytes

SAMN31975257 • SRS16126891 • All experiments • All runs

Organism: Pan troglodytes troglodytes

Library:

Name: 8720_2_dT5

Instrument: Sequel

Strategy: FL-cDNA

Source: TRANSCRIPTOMIC

Selection: Hybrid Selection

Layout: SINGLE

Runs: 1 run, 15,529 spots, 14M bases, 5.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR22838396	15,529	14M	5.8Mb	2023-01-10

ID:: 25883961

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